Before heading to work, I went to Brennan’s school to make sure that the audiovisual needs were met for his talk. I met Noriko on the way into the building and we were soon directed by his homeroom teacher to the computer room where Brennan’s talk was to take place. The room had been newly renovated to accommodate the school’s computers; it had new carpeting in addition to the dozens of new computers which lined the tables inside. I first tried hooking up a laptop that I had borrowed from Kiyoe to the projection system, but couldn’t get it to work. We finally settled on using the school’s main computer to open his talk, which I had also copied onto a memory stick, just in case.
Soon, Brennan’s entire 6th grade class filed into the room, about 30 kids in all. Brennan then spent the next 35 minutes talking about our trip, while Noriko translated his comments into Japanese. The kids all seemed quite interested in his travels, although most of their reactions were reserved for the question and answer session which followed. In addition to wanting to know what food as well as what city he had liked best, someone asked how long the trip had taken. The kids let out a gasp when Brennan had said that it had taken 3 weeks. That must have been an unheard of amount of time to be travelling (Kiyoe had admitted previously that, when I first told her that I wanted to take some time off in order to travel and see “the rest of Asia”, as I’d put it, she had pictured that I would be on the road about 5 days). The length question was soon followed by a child who wanted to know what the trip cost. Since Brennan had no idea, I answered the question with what must have been an unheard of amount of money to spend on one trip. With that, Brennan’s presentation was over- and I headed out to the monorail.
I arrived at work around 11 and immediately poured and ran a big gel in order to find out how my competitor-DNA containing chromosome reconstitutions had gone. By early afternoon, I had stained the gel and was pleased to see that the “glop” had disappeared from my experiments, as desired. As expected, the correct histone to DNA ratio had also dropped- so that a 1:1 ratio looked the best. One final test that remained was to remove the competitor DNA in order to digest the chromosome with an enzyme to ensure that everything was in order.
This proved to be difficult- Kiyoe gave me a protocol where one added magnesium to the samples (which compacts the chromosomes) and then spun them to recover the chromosomes and to leave behind the competitor DNA. When I did this, the chromosomes indeed disappeared from my spun samples, but were not to be found in the pelleted material either. Somehow I had lost them completely! I had completed these results around 7 pm, but it took another 30 minutes before I could discuss them with Kiyoe, who had been busy doing other things. Finally, at 8 pm, she suggested that I wait and look for them in the morning.
I arrived home about 8:45 to a fried chicken and mashed potato dinner which was waiting for me on the table. Trudy and the boys had gone ahead and eaten after I warned them that I was running very late. Trudy also opened a beer for me to have with dinner, she said that I could use one after a long day. We watched T.V. for a while, but soon turned in for bed.